mreB [2017-05-09 15:36:36]

cell shape-determining protein, forms filaments, the polymers control/restrict the mobility of the cell wall elongation enzyme complex, required for LytE activity

locus: BSU28030

pI: 4.90

mw: 35.79 kDa

protein length: 337 aa

gene length: 1011 bp

function: cell shape determination

product: cell shape-determining protein

essential: yes

synonyms: divIVB

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.2|Cell shape]
• [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.7|Membrane dynamics]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.2|Cell envelope stress proteins (controlled by SigM, V, W, X, Y)]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 2,860,735 → 2,861,748

Phenotypes of a mutant

• essential [Pubmed|12682299]
• the mutation can be suppressed by inactivation of ''[gene|A8EEC896B279CFDDE9C3A6F146A04EC822E33A4F|ponA]'', ''[gene|14ED1AF5038F43F3B151FCBABE6CFC5A2DA3AA6E|ptsI]'', ''[gene|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]'' [Pubmed|19192185], by overexpression of [protein|7150BABA5086D5E2EB8102E4901A216F43576282|GlmR] [Pubmed|21320184], or by addition of 5 mM magnesium to the growth medium [Pubmed|15752190]

The protein

Catalyzed reaction/ biological activity

• forms straight filaments in a heterologous system [Pubmed|21091501]
• polymerizes in the presence of millimolar divalent cations, binds and hydrolyzes GTP and ATP [Pubmed|19117023]
• involved in the organization of ϕ29 DNA replication machinery in peripheral helix-like structures [Pubmed|22420858]
• required for [protein|321F248C22D7283C0F3323F1F4069E36F8D7FE6C|LytE] activity [Pubmed|23869552]

Protein family

• ftsA/mreB family (according to Swiss-Prot)

Paralogous protein(s)

• [protein|6ED386D49F973C1F6B1076974F54275DD583C9D3|Mbl], [protein|40829C3632E9DCEB9E1C14A674B4B217F504CFE0|MreBH]

Modification

• acetylated at Lys-240, this is important for restricting cell wall growth and cell diameter [Pubmed|27376153]

Structure

• [PDB|1JCF] (from ''Thermotoga maritima'', 57% identity) [Pubmed|11544518]

[SW|Localization]

• during logarithmic growth, [protein|A4C8719E06F774A6EB4D79757CC79CF89E453A54|MreB] forms discrete patches that move processively along peripheral tracks perpendicular to the cell axis [Pubmed|21636744]
• forms transverse bands as cells enter the stationary phase [Pubmed|21636744]
• forms antiparallel double filaments [Pubmed|24843005]
• close to the inner surface of the cytoplasmic membrane [Pubmed|16950129]
• reports on helical structures formed by MreB [Pubmed|16950129,20566861] seem to be misinterpretation of data [Pubmed|21636744]
• normal localization depends on the presence of glucolipids, [protein|A4C8719E06F774A6EB4D79757CC79CF89E453A54|MreB] forms irregular clusters in an ''[gene|7A606B8E952AE8CA4F9A62008BA4B156725BB5B5|ugtP]'' mutant [Pubmed|22362028]
• during stationary phase: delocalized in the cytoplasm [Pubmed|26091431]
• during development of [SW|genetic competence]: co-localizes with polar clusters of the late competence protein [protein|41DFEF8A6BC7E34A6681C26D92F2B08DC120A957|ComGA] ([protein|41DFEF8A6BC7E34A6681C26D92F2B08DC120A957|ComGA] sequesters [protein|A4C8719E06F774A6EB4D79757CC79CF89E453A54|MreB]) [Pubmed|26091431]

Expression and Regulation

Operons

• genes: [gene|A4C8719E06F774A6EB4D79757CC79CF89E453A54|mreB]-[gene|6A14D0C7FD94A838BAB3B74AA100EA678F1E4E34|mreC]-[gene|231C30963AFB863424C087CB722446D319976D65|mreD]-[gene|8C94C9598A823A8405B3E1FA0124E21D90845B8E|minC]-[gene|37DAD42A391E2FC506225EAF91B8F21629A401DF|minD]
  description: [Pubmed|8459776]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, promoter p1, upstream of [protein|49D996C9AED44A820A214C3DF60AB1B6E1508DEA|Maf] [Pubmed|26091431], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • [protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM]: sigma factor, promoter p2, within [protein|49D996C9AED44A820A214C3DF60AB1B6E1508DEA|Maf] [Pubmed|18179421], in [regulon|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM regulon]
  • [protein|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|SigH]: sigma factor, promoter p4, upstream of [protein|8C94C9598A823A8405B3E1FA0124E21D90845B8E|MinC] [Pubmed|8459776], in [regulon|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|SigH regulon]

  regulatory mechanism

  • [protein|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|ComK]: activation, [Pubmed|26091431], in [regulon|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|ComK regulon]

  regulation

  • constitutively expressed [Pubmed|23701187]

• genes: [gene|49D996C9AED44A820A214C3DF60AB1B6E1508DEA|maf]-[gene|2BD11D71AA6D9EFAD8A209C8813CC8B059180C84|radC]-[gene|A4C8719E06F774A6EB4D79757CC79CF89E453A54|mreB]-[gene|6A14D0C7FD94A838BAB3B74AA100EA678F1E4E34|mreC]-[gene|231C30963AFB863424C087CB722446D319976D65|mreD]-[gene|8C94C9598A823A8405B3E1FA0124E21D90845B8E|minC]-[gene|37DAD42A391E2FC506225EAF91B8F21629A401DF|minD]
  description: [Pubmed|22383849]

  sigma factors

  • [protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM]: sigma factor, promoter p2 within [protein|49D996C9AED44A820A214C3DF60AB1B6E1508DEA|Maf] [Pubmed|18179421], in [regulon|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM regulon]
  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, promoter p1, upstream of [protein|49D996C9AED44A820A214C3DF60AB1B6E1508DEA|Maf] [Pubmed|26091431], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|ComK]: activation, [Pubmed|11948146,11918817,21564336], in [regulon|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|ComK regulon]

  regulation

  • constitutively expressed [Pubmed|23701187]

Biological materials

two-hybrid system

• ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in the labs of [SW|Jeff Errington] and [SW|Boris Görke]

Antibody

• available in the [SW|Jeff Errington] and [SW|Peter Graumann] labs

Labs working on this gene/protein

• [SW|Jeff Errington], Newcastle University, UK [http://www.ncl.ac.uk/camb/staff/profile/jeff.errington homepage]
• [SW|Peter Graumann], Freiburg University, Germany [http://www.biologie.uni-freiburg.de/data/bio2/graumann/index.htm homepage]

References

Reviews

• 17506674,17158703,15037301,21725336,20825347,21047262,22166997,22154164,22652894,21371139,25578957,25957405

Localization

• 17064365,20566861,21636744,21636745,22069484,23783036,24010660,24843005,25402772,25676310

Other original publications

• 15752190,11290328,12809607,1400224,7836311,16950129,19192185,19117023,19643765,19654094,19659933,8459776,11544518,20133608,21091501,21320184,18179421,21964069,22343529,22362028,22420858,21926231,23879732,23869552,24261876,24603761,26091431,17880425,20233306,27215790,27376153